Description
This Eagle Biosciences Fecal Adenovirus Antigens ELISA Assay Kit is designed, developed and produced for the qualitative measurement of Adenovirus Antigens in test specimen. The assay utilizes the microplate-based enzyme immunoassay technique by coating highly purified antibody onto the wall of microtiter well. Assay controls and fecal specimen, as well as HRP-conjugated monoclonal antibody that specifically recognizes the inner capsid protein of the Adenoviruses are added to microtiter wells of microplate that was coated with a highly purified polyclonal anti-Adenovirus antibody on its wall. After an incubation period an immunocomplex of “Anti-Adenovirus Antibody – Adenovirus Antigens – HRP-conjugated Anti-Adenovirus Tracer Antibody” is formed if there is Adenovirus Antigens present in the test sample. The unbound tracer antibody and other protein or buffer matrix are removed in the subsequent washing step. HRP-conjugated tracer antibody bound to the well is then incubated with a substrate solution in a timed reaction and then measured in a spectrophotometric microplate reader. The enzymatic activity of the tracer antibody bound to Adenovirus captured on the wall of each microtiter well is directly proportional to the amount of Adenovirus Antigens level in each test specimen